Dr. Pawan Kumar Doharey

Assistant Professor & Research Scientist
Ajitmal, IN.

About

Highly accomplished Assistant Professor and Research Scientist with over 8 years of postdoctoral and project leadership experience in Biotechnology, specializing in drug target discovery, structural biology, gene editing, and RNA editing. Recognized for significant contributions to filarial parasite biochemistry, trypanosome RNA editing, and COVID-19 drug repurposing, evidenced by 20 research articles, 2 review articles, 2 book chapters, and a Google Scholar profile with 418 citations, an h-index of 12, and an i10-index of 16.

Work

Deen Dayal Upadhyay Gorakhpur University
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Assistant Professor

Gorakhpur, Uttar Pradesh, India

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Summary

Leads academic instruction and research initiatives for postgraduate biotechnology students at a prominent state university.

Highlights

Delivered advanced lectures and practical sessions to postgraduate biotechnology students, fostering critical thinking and research skills for approximately 50+ students per year.

Mentored and guided numerous postgraduate students in their research projects, contributing to their academic success and scientific development.

Contributed to departmental curriculum development and academic administrative duties, ensuring high educational standards and smooth operational flow.

Translational Health Science and Technology Institute
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Project Scientist-II

Faridabad, Haryana, India

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Summary

Directed and executed complex research projects focused on translational health, delivering critical experimental data and analysis.

Highlights

Managed and executed complex research projects, ensuring adherence to scientific protocols and delivering critical experimental data within an 11-month tenure.

Applied advanced biochemical and molecular biology techniques to investigate disease mechanisms, contributing to the institute's research pipeline.

Collaborated effectively with interdisciplinary teams, accelerating research progress and facilitating knowledge exchange in a fast-paced environment.

University of Allahabad
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Postdoctoral Researcher

Allahabad, Uttar Pradesh, India

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Summary

Conducted independent biochemical research, advancing understanding of molecular mechanisms and contributing to significant findings.

Highlights

Spearheaded independent research in biochemistry, resulting in 6 peer-reviewed research articles and 1 review article, significantly advancing scientific understanding during a 3-year tenure.

Conducted extensive *in silico* studies for drug repurposing against SARS-CoV-2 and gastric cancer, identifying potential therapeutic compounds.

Utilized advanced techniques in protein analysis, enzyme kinetics, and molecular docking to elucidate structure-function relationships of various biomolecules.

Texas A&M University
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Postdoctoral Researcher

College Station, Texas, US

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Summary

Executed cutting-edge biochemical and biophysical research, contributing to significant advancements in understanding complex biological systems.

Highlights

Conducted pioneering research on RNA editing helicase 2 subcomplex in Trypanosome holo-editosomes, publishing 2 high-impact research articles and 1 review article in leading journals.

Applied sophisticated biophysical and molecular biology methodologies to unravel site-specific and substrate-specific control of mRNA editing, contributing to fundamental biological insights.

Collaborated with international research teams, presenting findings at conferences and contributing to a book chapter on RNA metabolism in mitochondria.

Education

Banaras Hindu University
Varanasi, Uttar Pradesh, India

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Ph.D.

Biochemistry

Banaras Hindu University
Varanasi, Uttar Pradesh, India

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Master of Science (M.S.)

Biochemistry

Awards

ICMR-Junior Research Fellowship

Awarded By

Indian Council of Medical Research

Secured a competitive national fellowship to support biomedical research, demonstrating high potential in the field.

CSIR-Junior Research Fellowship

Awarded By

Council of Scientific and Industrial Research and University Grants Commission (CSIR-UGC)

Awarded a prestigious national fellowship for doctoral research in scientific disciplines, recognizing exceptional academic merit.

NET-LS (National Eligibility Test for Lectureship)

Awarded By

Council of Scientific and Industrial Research and University Grants Commission (CSIR-UGC)

Qualified for eligibility as a lecturer in Indian universities and colleges, demonstrating high academic aptitude and subject mastery.

Publications

Biophysical and in-silico studies on the structure-function relationship of Brugia malayi protein disulfide isomerase.

Published by

Journal of Biomolecular Structure & Dynamics

Summary

Doharey PK, Verma P, Dubey A, Singh SK, Kumar M, Tripathi T, Alonazi M, Siddiqi NJ, Sharma B. J Biomol Struct Dyn. 2023 Apr 20:1-11. doi: 10.1080/07391102.2023.2201849. [I.F. 5.235]

Biochemical Characterization of Different Chemical Components of Parthenium hysterophorus and Their Therapeutic Potential against HIV-1 RT and Microbial Growth.

Published by

BioMed Research International

Summary

Jaiswal J, Doharey PK, Singh R, Tiwari P, Singh N, Kumar A, Gupta VK, Siddiqui AJ, Sharma B. Biomed Res Int. 2022 Apr 28;2022:3892352. doi: 10.1155/2022/3892352. PMID: 35528165; PMCID: PMC9071890. [I.F. 2.583]

Pharmacological Chemistry and Biomedical Implications of Chemical Ingredients from Parthenium hysterophorus.

Published by

Current Topics in Medicinal Chemistry

Summary

Jaiswal J, Singh N, Gupta VK, Doharey PK, Siddiqi NJ, Sharma B. Curr Top Med Chem. 2022 Mar 7. doi: 10.2174/15680266226662203071450 27. Epub ahead of print. PMID: 35255797. [I.F. 3.295]

Repurposing of gastric cancer drugs against COVID-19.

Published by

Computers in Biology and Medicine

Summary

Sonkar C*, Doharey PK*, Rathore AS, Singh V, Kashyap D, Sahoo AK, Mittal N, Sharma B, Jha HC. Comput Biol Med. 2021 Oct; 137:104826. doi: 10.1016/j.compbiomed.2021.104826. Epub 2021 Sep 6. PMID: 34537409; PMCID: PMC8420180. [I.F. 6.698]

Modulating catalytic activity of human topoisomerase II α enzyme by fluorescent gold nanoclusters.

Published by

International Journal of Biological Macromolecules

Summary

Dubey A, Singh V*, Doharey PK*, Sk MP, Samanta SK, Nema V, Sharma B, Varadwaj PK, Sahoo AK. Int J Biol Macromol. 2021 Feb 15; 170:523-531. doi: 10.1016/j.ijbiomac.2020.12.129. Epub 2020 Dec 30. PMID: 33387542. [I.F. 8.025]

In silico study indicates antimalarials as direct inhibitors of SARS-CoV-2-RNA dependent RNA polymerase.

Published by

Journal of Biomolecular Structure & Dynamics

Summary

Doharey PK, Singh V, Gedda MR, Sahoo AK, Varadwaj PK, Sharma B. J Biomol Struct Dyn. 2021 Jan 21:1-18. doi: 10.1080/07391102.2021.1871956. Epub ahead of print. PMID: 33475021; PMCID: PMC7842134. [I.F. 5.235]

Site-specific and substrate-specific control of accurate mRNA editing by a helicase complex in trypanosomes.

Published by

RNA

Summary

Kumar V, Ivens A, Goodall Z, Meehan J, Doharey PK, Hillhouse A, Hurtado DO, Cai JJ, Zhang X, Schnaufer A, Cruz-Reyes J. RNA. 2020 Dec;26(12):1862-1881. doi: 10.1261/rna.076513.120. Epub 2020 Sep 1. PMID: 32873716; PMCID: PMC7668249. [I.F. 4.942]

Protein features for assembly of the RNA editing helicase 2 subcomplex (REH2C) in Trypanosome holo-editosomes.

Published by

PLoS One

Summary

Kumar V*, Doharey PK*, Gulati S, Meehan J, Martinez MG, Hughes K, Mooers BHM, Cruz-Reyes J. PLoS One. 2019 Apr 29;14(4): e0211525. doi: 10.1371/journal.pone.0211525. PMID: 31034523; PMCID: PMC6488192. [I.F. 3.752]

Dynamic RNA holo-editosomes with subcomplex variants: Insights into the control of trypanosome editing.

Published by

Wiley Interdisciplinary Reviews: RNA

Summary

Cruz-Reyes J, Mooers BHM, Doharey PK, Meehan J, Gulati S. Wiley Interdiscip Rev RNA. 2018 Nov;9(6): e1502. doi: 10.1002/wrna.1502. Epub 2018 Aug 12. PMID: 30101566; PMCID: PMC6185801. [I.F. 9.957]

Control Mechanisms of the Holo-Editosome in Trypanosomes in RNA Metabolism in Mitochondria.

Published by

Springer, Cham

Summary

Jorge Cruz-Reyes, Blaine H.M. Mooers, Vikas Kumar, Pawan K. Doharey, Joshua Meehan, Luenn Chhapro. Springer, Cham, 978-3-319- 78189-1. 125-144, 2018.

Molecular cloning and characterization of protein disulfide isomerase of Brugia malayi, a human lymphatic filarial parasite.

Published by

EXCLI Journal

Summary

Verma P, Doharey PK, Yadav S, Omer A, Singh P, Saxena JK. EXCLI J. 2017 Jun 1; 16:824-839. doi: 10.17179/excli2017-214. PMID: 28827998; PMCID: PMC5547380. [I.F. 4.022]

Molecular cloning, purification and characterization of Brugia malayi phosphoglycerate kinase.

Published by

Protein Expression and Purification

Summary

Kumar R, Doharey PK, Saxena JK, Rathaur S. Protein Expr Purif. 2017 Apr; 132:152-163. doi: 10.1016/j.pep.2017.02.005. Epub 2017 Feb 10. PMID: 28192198. [I.F. 1.695]

Insights into the structure-function relationship of Brugia malayi thymidylate kinase (BmTMK).

Published by

International Journal of Biological Macromolecules

Summary

Doharey PK, Singh SK, Verma P, Verma A, Rathaur S, Saxena JK. Int J Biol Macromol. 2016 Jul; 88:565-71. doi: 10.1016/j.ijbiomac.2016.04.004. Epub 2016 Apr 1. PMID: 27044348. [I.F. 8.025]

NADP⁺ binding effects tryptophan accessibility, folding and stability of recombinant B. malayi G6PD.

Published by

International Journal of Biological Macromolecules

Summary

Verma A, Chandra S, Suthar MK, Doharey PK, Siddiqi MI, Saxena JK. Int J Biol Macromol. 2016 Apr; 85:645-54. doi: 10.1016/j.ijbiomac.2015.12.087. Epub 2016 Jan 4. PMID: 26763177. [I.F. 8.025]

Designing, synthesis of selective and high-affinity chalcone-benzothiazole hybrids as Brugia malayi thymidylate kinase inhibitors: In vitro validation and docking studies.

Published by

European Journal of Medicinal Chemistry

Summary

Sashidhara KV, Avula SR, Doharey PK, Singh LR, Balaramnavar VM, Gupta J, Misra-Bhattacharya S, Rathaur S, Saxena AK, Saxena JK. Eur J Med Chem. 2015 Oct 20; 103:418-28. doi: 10.1016/j.ejmech.2015.09.004. Epub 2015 Sep 8. PMID: 2638312. [I.F. 7.1]

Synthesis, molecular docking and Brugia malayi thymidylate kinase (BmTMK) enzyme inhibition study of novel derivatives of [6]-shogaol.

Published by

European Journal of Medicinal Chemistry

Summary

Singh VK, Doharey PK, Kumar V, Saxena JK, Siddiqi MI, Rathaur S, Narender T. Eur J Med Chem. 2015 Mar 26;93:74-82. doi: 10.1016/j.ejmech.2015.01.035. Epub 2015 Jan 20. PMID: 25659753. [I.F. 7.1]

Operative conversions of 3-carboxy-4-quinolones into 3-nitro-4-quinolones via ipso-nitration: potential antifilarial agents as inhibitors of Brugia malayi thymidylate kinase.

Published by

RSC Advances

Summary

CS Azad, VM Balaramnavar, IA Khan, Doharey PK, JK Saxena, A K Saxena. RSC Advances 2015, 5 (100), 82208-82214. doi.org/10.1039/C5RA18036H. [I.F. 4.036]

Identification of novel PTP1B inhibitors by pharmacophore-based virtual screening, scaffold hopping and docking.

Published by

European Journal of Medicinal Chemistry

Summary

Balaramnavar VM, Srivastava R, Rahuja N, Gupta S, Rawat AK, Varshney S, Chandasana H, Chhonker YS, Doharey PK, Kumar S, Gautam S, Srivastava SP, Bhatta RS, Saxena JK, Gaikwad AN, Srivastava AK, Saxena AK. Eur J Med Chem. 2014 Nov 24; 87:578-94. doi: 10.1016/j.ejmech.2014.09.097. Epub 2014 Oct 2. PMID: 25299681. [I.F. 7.1]

In silico and in vitro studies on the protein-protein interactions between Brugia malayi immunomodulatory protein calreticulin and human C1q.

Published by

PLoS One

Summary

Yadav S, Gupta S, Selvaraj C, Doharey PK, Verma A, Singh SK, Saxena JK. PLoS One. 2014 Sep 3;9(9): e106413. doi: 10.1371/journal.pone.0106413. PMID: 25184227; PMCID: PMC4153637. [I.F. 3.752]

Molecular cloning and characterization of Brugia malayi thymidylate kinase.

Published by

Acta Tropica

Summary

Doharey PK, Suthar MK, Verma A, Kumar V, Yadav S, Balaramnavar VM, Rathaur S, Saxena AK, Siddiqi MI, Saxena JK. Acta Trop. 2014 May; 133:83-92. doi: 10.1016/j.actatropica.2014.02.003. Epub 2014 Feb 17. PMID: 24556140. [I.F. 3.222]

Behavior of Plasmodium falciparum purine nucleoside phosphorylase in macromolecular crowded environment.

Published by

International Journal of Biological Macromolecules

Summary

Suthar MK, Doharey PK, Verma A, Saxena JK. Int J Biol Macromol. 2013 Nov; 62:657-62. doi: 10.1016/j.ijbiomac.2013.09.036. Epub 2013 Oct 1. PMID: 24095713. [I.F. 8.025]

Molecular cloning and characterization of glucose-6-phosphate dehydrogenase from Brugia malayi.

Published by

Parasitology

Summary

Verma A, Suthar MK, Doharey PK, Gupta S, Yadav S, Chauhan PM, Saxena JK. Parasitology. 2013 Jun;140(7):897-906. doi: 10.1017/S0031182013000115. Epub 2013 Mar 18. PMID: 23506961. [I.F. 2.783]

Single tryptophan of disordered loop from Plasmodium falciparum purine nucleoside phosphorylase: involvement in catalysis and microenvironment.

Published by

Applied Biochemistry and Biotechnology

Summary

Suthar MK, Verma A, Doharey PK, Singh SV, Saxena JK. Appl Biochem Biotechnol. 2013 Jun;170(4):868-79. doi: 10.1007/s12010-013-0228-9. Epub 2013 Apr 25. PMID: 23615735. [I.F. 2.431]

Filariasis: Biochemistry and Chemotherapeutic Targets.

Published by

Nova Publisher USA

Summary

Jitendra Kumar Saxena, Anita, Manish Kumar Suthar, Pawan K. Doharey, Alok R. Singh. In Nematodes: Morphology, Functions and Management Strategies. Publisher: Nova Publisher USA. ISBN 978-1-61470-784-4. 83-107, 2013.

Languages

English
Hindi

Skills

Drug Discovery & Development

Drug Target Identification, Chemotherapeutic Targets, Medicinal Chemistry, Pharmacophore Modeling, Virtual Screening, Molecular Docking, Enzyme Inhibition Studies.

Structural Biology

Protein Structure-Function Analysis, Biophysical Studies, Macromolecular Interactions, Protein Characterization.

Vaccine Development

Immunology, Antigen Identification, Pathogen Biology.

Gene Editing

CRISPR-Cas Systems, Genetic Engineering, Molecular Cloning.

RNA Editing

RNA Metabolism, Holo-editosomes, Trypanosome Biology, mRNA Processing.

Molecular Biology

Molecular Cloning, Gene Expression, PCR, DNA/RNA Isolation, Protein Expression & Purification.

Biochemistry

Enzyme Kinetics, Protein Biochemistry, Metabolic Pathways, Spectroscopy, Chromatography.

Bioinformatics & In Silico Analysis

Molecular Docking, Virtual Screening, Homology Modeling, Sequence Analysis, Computational Biology.

Academic & Research Leadership

Postgraduate Teaching, Curriculum Development, Student Mentorship, Research Project Management, Scientific Writing, Grant Writing.

Data Analysis

Statistical Analysis, Scientific Data Interpretation, Experimental Design.